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Objective@#To analyze the correlation between severe pneumonia and inflammatory factors and TBX21 locus rs16947078.@*Methods@#From March 2017 to March 2019, in the Fourth People's Hospital of Wenling, 71 patients with severe pneumonia were selected, and 83 patients with general pneumonia were selected during the same period.The levels of interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-18 (IL-18) and tumor necrosis factor-alpha (TNF-α) were detected by enzyme-linked immunosorbent assay (ELISA). The genotype and gene distribution of rs16947078 at TBX21 locus were detected by real-time fluorescence quantitative PCR.@*Results@#The score of CPIS in the severe pneumonia group was higher than that in the common pneumonia group [(6.57±1.20)points and (4.93±1.05)points] (t=9.045, P<0.05). The serum levels of IL-6 [(65.42±13.25)ng/L], IL-8 [(457.87±45.36)ng/L], IL-18 [(87.59±15.46)ng/L] and TNF-α[(58.98±13.25)ng/L] in the severe pneumonia group were higher than those in the general pneumonia group [(36.71±8.98)ng/L, (218.98±32.45)ng/L, (54.37±12.10)ng/L and (37.54±7.90)ng/L] (t=15.926, 37.959, 14.946, 12.394, all P<0.05). The G distribution of rs16947078 allele at TBX21 locus in the severe pneumonia group (40.85%) was higher than that in the normal pneumonia group (18.07%) (χ2=9.724, P<0.05). The GG (36.62%) of TBX21 locus rs16947078 in the severe pneumonia group was higher than that in the common pneumonia group (12.05%), while the AA (52.11%) of TBX21 locus in the severe pneumonia group was lower than that in the common pneumonia group (72.29%) (χ2=12.898, 6.682, P<0.05).@*Conclusion@#The levels of inflammatory factors IL-6, IL-8, IL-18 and TNF-α in patients with severe pneumonia aresignificantly increased, and the TBX21 locus rs16947078 allele A may be a susceptible gene for severe pneumonia.
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Objective To evaluate the influence of cytotoxin-associated gene A (CagA)to the differentiation of helper T lymphocytes in chronic gastritis. Methods Eighty patients with chronic gastritis were included in this study. The serum antibody against CagA was detected by the enzyme-linked immunosorbant assay (ELISA). Patients were divided into CagA-positive group and CagA-negative group according to the results of ELISA. Pathologic changes in gastric mucus were respectively analyzed. In addition, the expressions of nuclear transcription factors in gastric mucus including TBX 21, GATA-3, FoxP3 and Rorγt were detected by PCR and Western blot assay. Results There were 52 patients in CagA-positive group and 28 patients in CagA-negative group. The gastric inflammation was more serious in CagA-positive group than that in CagA-snegative group. There was no significant difference in Hp density between two groups. The expressions of GATA-3 and FoxP3 were much higher, while the transcription and protein expression levels of TBX21 and Rorγt were significantly lower in CagA-positive group than those in CagA-negative group. Conclusion Although the inflammation in gastric mucus was more serious in CagA-positive patients, Hp can not be effectively eliminated,which may relate to the differentiation of Th0 into Th2/Treg cells.
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OBJECTIVE: Behcet's disease (BD) is a chronic systemic inflammatory disease with unknown etiology. A number of clinical and laboratory findings suggest a strongly polarized Th1 immune response in BD. T-bet is a newly identified Th1 specific T-box transcription factor selectively expressed in Th1 cells. However, it is not yet clear whether the T-bet protein is involved in the proposed Th1-mediated pathogenesis of BD at the transcriptional level. Therefore, this study investigated the potential associations of two single nucleotide polymorphisms (SNPs) at positions -99 (C/G) and -1993 (T/C) in the exon and promoter regions of the TBX21 gene with susceptibility to BD in the Korean population. METHODS: 105 patients with BD and 105 healthy controls were studied. All subjects were genotyped using restriction fragment length polymorphism analysis. The genotypes of the two groups were compared with the chi-square or Fisher's exact tests. RESULTS: The genotypic and allelic distributions of the two SNPs did not differ significantly between the two groups. Furthermore, no associations between the polymorphisms and clinical manifestations were found, except a central nervous system manifestation and arthritis. Furthermore, no associations between the polymorphisms and severity were identified. CONCLUSION: TBX21 gene polymorphisms were not associated with susceptibility, clinical manifestations, or severity of BD in the Korean population.
Subject(s)
Humans , Arthritis , Central Nervous System , Exons , Genotype , Polymorphism, Restriction Fragment Length , Polymorphism, Single Nucleotide , Promoter Regions, Genetic , Th1 Cells , Transcription FactorsABSTRACT
TBX21 (T-bet) is a member of the T-box family of transcriptional factors that contain a conserved DNA binding domain. TBX21 is a critical regulator of the commitment to the Th1 lineage and IFN-gamma production. Th1 and Th2 cells cross-regulate the differentiation of each other, and in this way TBX21 could be an attractive candidate gene for treating autoimmune disease such as rheumatoid arthritis (RA). In present study, we analyzed the genotypic frequencies of six polymorphisms of the TBX21 gene between the 367 RA patients and the 572 healthy controls. We showed that the g.-1514T>C and c.99C>G polymorphisms are suggestively associated with RA susceptibility. It is interesting that the genotypic frequencies of the TBX21 polymorphisms (g.-1514T>C and c.2103A>C) in the male RA patients were significantly different from the male control group (P = 0.0016 and 0.045, respectively). We also found that the g.-1514T>C and c.2103A>C polymorphisms of the TBX21 gene in the male RA patients have significant association with the levels of anti-CCP (P = 0.05) and rheumatoid factor (P = 0.03), respectively. These results suggest that the polymorphisms of the TBX21 gene might be associated with the susceptibility to male RA patients.